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KMID : 0378019800230100111
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New Medical Journal
1980 Volume.23 No. 10 p.111 ~ p.120
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Analysis of Blood Culture
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Abstract
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Detecting the presence of micro-organism in blood among the unknown fever patients is .an important function of the clinical microbiology laboratory, but the study also include pathologic leukocytosis and sign of shock after surgery, trauma, after tissue injury, prolonged administration of antibiotics and immunosuppressive drugs and postirradiation :states.
But many factors such as the bacterial status, the number of living micro-organisms in blood, the condition of cultivation technic, selection of media, time of blood collection, bacteriostatic substances, ratio of blood volume and media and concentration of antibiotic level in blood directly influence bacterial growth in the media, beta-lysin, hematin, leukin, lysozyme, phagocytin, plakin, natural antibodies and complement which is well known .and documented as a bacteriostatic substances in the blood.
The introduction and widespread use of chemotherapeutic and antibiotic agents has resulted in profound changes in the number and character of infections that are being encountered and also, it makes confusion for interpretation of the result of bacterial ccontamination during blood sampling and routine course of media inoculation.
Blood cultures are often essential in diagnosis and prognosis of disease. Frequently, however, the results obtained are difficult to interpret and correlate with the clinical state of the patient. Limitation and confusion resulting may be attributable to (a) contamination due to faulty technique in the drawing of blood in laboratory procedures, (b) preconceived ideas of bacterial "nonpathogenecity" and (c) variations in technique of blood cultures.
Total analysis of 6535 cases of blood cultures from May 1972 to July 1978 and bacterial
identifications are obtained 871 strains at Han Yang University Hospital. The Results were as follows:
1. Positive blood culture is -13.3%´ (9.4-18.940)
2. Summer (June _August) and winter (December-January) time show two peak with 17.0%, 18.6´%0, 15.9% and 14.7% respectively.
3. Adults (20-39 years old) and neonatal baby are higher incidence (21.5% and 13.9%) in septicemia.
4. The highest incidence is typhoid fever 52.0%.
5, Typhoid fever: Geopen, panimycin, streptomycin and kanamycin have over 90%´ in sensitivity, but chloramphenicol has only 80.9%.
6. Staph. aureus: cloxacillin, panimycin, panimycin and minocin have almost over 94%% in sensitivity, but ampicillin has only 3.6%.
7. Alpha and beta hemolytic streptococcus and strep. pneumoniae: ampicillin, geopen, cefamezin and penicillin have over 80% in sensitivity, but colistin has only below 50%.
8. Gamma streptococcus: only rninocin has 80% in sensitivity, but penicillin, ampicillin and erythromycin have 55% resistance in sensitivity.
9. Pseudomonas: except geopen (45%), all antibiotics have resistance.
10. Proteus: only geopen has 100% sensitivity, but penicillin, ampicillin and cefamezin have 100% resistance.
11. E. Coli: only chloramphenicol has 81% sensitivity, but lincocin, and penicillin have 65% resistance in sensitivity.
12. Coliform bacilli: panimycin has 100% sensitivity, otherwise penicillin and ampicillin have 100% resistance.
13. N. meningitidis: penicillin, ampicillin and cefamezin and geopen have 1001, sensitivity, otherwise no antibiotics have resistance.
14. Enteric gram negative microorganism: generally geopen, chloramphenicol and gentamycin have.higher sensitivity.
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KEYWORD
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